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Analytical methods in strigolactone research.

Rostislav Halouzka1, Sanja Ćavar Zeljković1,2, Bořivoj Klejdus3,4

  • 1Centre of Region Haná for Biotechnological and Agricultural Research, Department of Phytochemistry, Faculty of Science, Palacky University, Šlechtitelů 27, 78371 Olomouc, Czechia.

Plant Methods
|June 10, 2020
PubMed
Summary

Strigolactones (SLs), crucial plant hormones, require precise chemical analysis for understanding their roles. This review details methods for identifying and quantifying SLs in plants, aiding future research.

Keywords:
DESI-MSDeterminationGC–MSHR-DART-MSIsolationLC–MS/MSStrigolactones

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Area of Science:

  • Plant Biology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Strigolactones (SLs) are vital plant hormones regulating root development, plant-microbe symbioses, and parasitic plant germination.
  • SLs are produced via the carotenoid biosynthetic pathway and exist in very low concentrations, necessitating sensitive detection methods.
  • Understanding SL biochemistry and physiology relies heavily on accurate chemical analysis.

Purpose of the Study:

  • To review and discuss key issues in the identification and quantification of strigolactones from plant samples.
  • To evaluate the advantages and disadvantages of various strigolactone analysis protocols.
  • To propose guidelines for minimizing sample loss during isolation and purification.

Main Methods:

  • Discussion of established hyphenated techniques like Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
  • Highlighting newer ambient mass spectrometry techniques such as High-Resolution Desorption Electrospray Ionization-Mass Spectrometry (HR-DART-MS) and Desorption Electrospray Ionization-Mass Spectrometry (DESI-MS).
  • Emphasis on methods requiring minimal sample preparation.

Main Results:

  • Various analytical techniques exist for strigolactone analysis, each with specific benefits and limitations.
  • Hyphenated techniques offer robust quantification, while ambient MS techniques provide rapid analysis with simple sample preparation.
  • Effective isolation and purification protocols are critical for accurate final analysis.

Conclusions:

  • Accurate identification and quantification of strigolactones are essential for advancing plant science.
  • Selecting appropriate analytical methods, considering sample preparation and technique sensitivity, is crucial.
  • Emerging ambient MS techniques show significant promise for strigolactone research due to their simplicity and speed.