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Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
Published on: February 7, 2021
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Multilayer Injectable Hydrogel System Sequentially Delivers Bioactive Substances for Each Wound Healing Stage
Zhijie Ma1,2, Wei Song3,4, Yaohua He3,4
1Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, China.
ACS Applied Materials & Interfaces
|June 10, 2020
Summary
This study introduces a novel injectable hydrogel system that sequentially delivers therapeutic agents to optimize wound healing. The system promotes tissue regeneration by managing inflammation, vascularization, and preventing fibrosis.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Drug Delivery Systems
Background:
- Wound healing is a complex, multi-stage process crucial for tissue regeneration.
- Current drug delivery systems often fail to address the distinct requirements of each healing phase.
- A need exists for advanced delivery systems capable of sequential, stage-specific therapeutic release.
Purpose of the Study:
- To develop and evaluate a multilayer injectable hydrogel system for sequential drug delivery in wound healing.
- To design a system that precisely matches the bioactive molecule release profile to the timeline of wound healing phases.
- To enhance overall tissue regeneration and minimize scarring.
Main Methods:
- Fabrication of an injectable sodium alginate/bioglass (SA/BG) composite hydrogel.
- Encapsulation of cell-conditioned medium (CM) within SA microparticles (SACM).
- Further encapsulation of pirfenidone (PFD)-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres (PLGAPFD) within SACM microparticles, forming a multilayer system (SA/BG-SACM-PLGAPFD).
Main Results:
- The SA/BG component released ions to modulate inflammation (days 1-3).
- SACM microparticles released CM to promote vascularized granulation tissue formation (days 2-7).
- PLGAPFD microspheres released PFD to inhibit fibrosis and scar formation (days 8-20).
- The system demonstrated efficacy in a diabetic mouse skin damage model, restraining inflammation, accelerating healing, and inhibiting fibrosis.
Conclusions:
- The developed SA/BG-SACM-PLGAPFD hydrogel system effectively delivers bioactive molecules sequentially, supporting distinct wound healing phases.
- This multilayer system enhances skin regeneration by controlling inflammation, promoting vascularization, and preventing fibrosis.
- The adaptable nature of this injectable biomaterial system offers broad potential for applications in regenerative medicine.
