Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

11.6K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
11.6K
Antimicrobial Proteins01:23

Antimicrobial Proteins

12.8K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
12.8K
Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

9.8K
Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
9.8K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.8K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of C-Terminal Amide <i>N</i>-Derivatization on the Conformational Dynamics and Antimitotic Activity of Cemadotin Analogues.

Molecules (Basel, Switzerland)·2026
Same author

Creating unimolecular multivalent diversity in protein conjugates via the Passerini multicomponent bioconjugation with isocyanoproteins.

Communications chemistry·2026
Same author

Exploration of the Tertiary Amide Chemical Space of Dolastatin 15 Analogs Reveals New Insights into the Structure-Anticancer Activity Relationship.

ChemMedChem·2025
Same author

Heterogeneous Catalysis Expands the Toolbox for Chemoselective Peptide Derivatization and Labeling.

Journal of the American Chemical Society·2025
Same author

Development of bombesin-tubulysin conjugates using multicomponent chemistry to functionalize both the payload and the homing peptide.

Frontiers in pharmacology·2024
Same author

Effect of O-Acetylation on the Antigenicity and Glycoconjugate Immunogenicity of the Streptococcus Pneumoniae Serotype 7F Capsular Polysaccharide.

Chembiochem : a European journal of chemical biology·2024

Related Experiment Video

Updated: Dec 18, 2025

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

12.2K

Macrocyclic Iminopeptides Diversify To Better Target Proteins.

Leslie Reguera1, Daniel G Rivera1

  • 1Faculty of Chemistry, University of Havana, Zapata y G, Havana, 10400, Cuba.

Chemmedchem
|June 10, 2020
PubMed
Summary

Researchers developed a novel method for creating diverse cyclic peptides by derivatizing macrocyclic iminopeptides. This strategy enables the synthesis of unique peptide-natural product hybrids, called PepNats, for potential therapeutic applications.

Keywords:
cyclic peptidescycloaddition reactionsmacrocyclesprotein−protein interactions

More Related Videos

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

12.9K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

3.0K

Related Experiment Videos

Last Updated: Dec 18, 2025

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
08:48

Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation

Published on: January 26, 2016

12.2K
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

12.9K
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

3.0K

Area of Science:

  • Medicinal Chemistry
  • Organic Synthesis
  • Chemical Biology

Background:

  • Cyclic peptides are crucial in drug discovery due to their stability and unique structures.
  • Derivatization of macrocyclic iminopeptides is an underexplored area for generating molecular diversity.
  • Developing novel synthetic strategies is key to accessing conformationally diverse cyclic peptides.

Purpose of the Study:

  • To introduce a new complexity-generating method for synthesizing macrocyclic peptides.
  • To explore the derivatization of cyclic iminopeptides through 1,3-cycloaddition reactions.
  • To create a novel class of macrocyclic peptide/natural product hybrids (PepNats).

Main Methods:

  • On-resin synthesis of macrocyclic peptide/natural product hybrids (PepNats).
  • Utilizing 1,3-cycloaddition reactions for the derivatization of cyclic iminopeptides.
  • Employing peptide sequences that mimic protein hot loops for proof-of-concept studies.

Main Results:

  • Successful on-resin construction of a new family of macrocyclic peptide/natural product hybrids (PepNats).
  • Demonstrated the potential of 1,3-cycloaddition reactions for derivatizing cyclic iminopeptides.
  • PepNats were shown to mimic protein hot loops, indicating potential for protein-protein interaction modulation.

Conclusions:

  • The derivatization of cyclic iminopeptides offers a powerful strategy for expanding synthetic repertoires.
  • The developed method enables the creation of novel macrocyclic peptide ligands.
  • PepNats represent a promising new class of molecules for modulating protein-protein interactions and drug discovery.