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Related Concept Videos

Sulfur Assimilation01:20

Sulfur Assimilation

238
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
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Mass Spectrometry: Isotope Effect01:13

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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Stable isotopes to study sulfur amino acid utilization in broilers.

R M Suzuki1, L G Pacheco2, J C P Dorigam3

  • 1Department of Animal Science, São Paulo State University, Jaboticabal, 148830-900, Brazil.

Animal : an International Journal of Animal Bioscience
|June 11, 2020
PubMed
Summary
This summary is machine-generated.

Dietary methionine (Met) and cysteine (Cys) ratios impact broiler performance. A 50:50 Met:Cys ratio optimized broiler growth and feed conversion, indicating altered sulfur amino acid metabolism in broilers aged 42-56 days.

Keywords:
broilercysteinelabeled amino acidmethioninetranssulfuration

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Area of Science:

  • Animal Nutrition
  • Poultry Science
  • Amino Acid Metabolism

Background:

  • Dietary methionine (Met) and cysteine (Cys) are crucial total sulfur amino acids (TSAA) for broiler performance, particularly feather growth.
  • The necessity of dietary cyst(e)ine supplementation alongside methionine is debated, as methionine can be converted to cysteine.
  • Previous TSAA requirement studies often omitted free cyst(e)ine, and optimal Met:Cys ratios may vary with broiler age and methionine source.

Purpose of the Study:

  • To evaluate two synthetic methionine sources (dl-Met and l-Met).
  • To determine the optimal proportion of methionine and cysteine within the total sulfur amino acid (TSAA) requirement for broiler performance.
  • To investigate the impact of different Met:Cys ratios on TSAA metabolism in broilers.

Main Methods:

  • A performance assay with 1080 broilers (42-56 days) used a 5x2 factorial arrangement.
  • Diets featured varying Met:Cys ratios (44:56 to 52:48) while maintaining constant TSAA levels.
  • Metabolism studies used L-(15N)Met or L-(15N2)Cystine in diets with 44:56, 48:52, and 52:48 Met:Cys ratios.

Main Results:

  • No significant differences were found between dl-Met and l-Met sources for feed intake, body weight gain, or feed conversion ratio (FCR).
  • A 50:50 Met:Cys ratio numerically improved FCR.
  • Increasing Met:Cys ratios enhanced methionine conversion to cysteine and decreased methionine intermediate concentrations, indicating broiler adaptation.

Conclusions:

  • Broiler chickens adjust their sulfur amino acid metabolism in response to dietary Met:Cys proportions.
  • A 50:50 Met:Cys ratio is recommended for optimizing broiler performance between 42 and 56 days of age.
  • Methionine source did not significantly affect performance, but the ratio of Met to Cys is critical for efficient TSAA utilization.