Gelling behavior of bio-tofu coagulated by microbial transglutaminase combined with lactic acid bacteria
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Updated: Sep 21, 2026

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
Published on: January 1, 2016
Guangliang Xing1, Concetta Valeria Lucia Giosafatto2, Andrea Carpentieri2
1School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, Jiangsu Province, PR China; College of Food Science and Technology, Nanjing Agricultural University, Nan Jing 210095, Jiangsu Province, PR China; Department of Chemical Sciences, University of Naples Federico II, Via Cinthia, 80126 Naples, Italy.
Abstract:
The aim of this study was to investigate the gelling behavior of proteins in bio-tofu (soymilk-cow milk mixture gel) coagulated by microbial transglutaminase (MTGase) combined with lactic acid bacteria (LAB). It was shown that MTGase (3.0 U/g protein) treatment of soymilk-cow milk mixture (SCMM) could not induce gelation at 43℃ even if the incubation was lasting 4 h. However, the concomitant use of LAB (0.025 UC/L) along with MTGase could induce the formation of denser and finer gel network with smaller pores and higher storage modulus (G') compared to SCMM treated with only LAB. Electrophoresis and mass spectrometry results indicated that LAB improve MTGase-dependent polymerization of proteins. In addition, this study investigates the effect of LAB and MTGase treatment on the rheology behavior of the derived gel products. In general, the use of both bio-coagulants for the manufacture of a mixed protein gel, might open new horizons in the field of novel nutrional and functional foods.
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