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Related Experiment Videos

Cell separation in the buffy coat.

D W Sutton1, P C Chen, G W Schmid-Schönbein

  • 1AMES-Bioengineering, University of California, San Diego, La Jolla 92093.

Biorheology
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

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Buffy coat analysis rapidly determines blood cell counts. This study found minimal cell mixing in buffy coat layers, except for some erythrocytes, with distinct layers for platelets, lymphocytes, monocytes, granulocytes, and erythrocytes.

Area of Science:

  • Hematology
  • Cell Biology
  • Biophysics

Background:

  • The buffy coat, a thin layer in centrifuged blood, is used for rapid cell counting.
  • Understanding buffy coat layer purity is crucial for accurate hematological analysis.

Purpose of the Study:

  • To evaluate the separation and purity of blood cells within the buffy coat layers.
  • To investigate the composition of distinct buffy coat layers formed after density centrifugation.

Main Methods:

  • Density centrifugation of whole blood in microhematocrit tubes with floats.
  • Stereological analysis of electron micrographs to assess cell mixing.
  • Cell mass density determinations.

Main Results:

Related Experiment Videos

  • Normal blood separates into platelets, lymphocytes/monocytes, granulocytes, and erythrocytes.
  • Minimal intermixing (<2%) of cells observed, with exceptions for erythrocytes in specific layers.
  • Abnormal samples showed feathered interfaces and increased erythrocyte volume in granulocyte layers due to lowered erythrocyte density.

    • Conclusions:

    • Buffy coat centrifugation provides distinct blood cell layers with high purity.
    • Erythrocyte density variations can lead to altered layer composition in certain blood samples.
    • This method offers a rapid assessment of blood cell separation and purity.