Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

15.9K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
15.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Infrared free electron laser induced photodesorption of CO and N<sub>2</sub> from solid amorphous water at cryogenic temperatures.

Physical chemistry chemical physics : PCCP·2026
Same author

Structural determinants and biochemical characterization of LORELEI as a GPI-anchored protein.

Plant physiology·2025
Same author

Cryogenic action spectroscopy of the cyan fluorescent protein chromophore anion.

Physical chemistry chemical physics : PCCP·2025
Same author

IR-Induced CO Photodesorption from Pure CO Ice and CO on Amorphous Solid Water.

ACS earth & space chemistry·2025
Same author

Peptide signalling in flowering plants: insights into reproductive thermotolerance.

Journal of experimental botany·2025
Same author

Enhanced pollen tube performance at high temperature contributes to thermotolerant fruit and seed production in tomato.

Current biology : CB·2024
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Dec 18, 2025

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
09:17

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

648

Workflow to Characterize Mutants with Reproductive Defects.

Jennifer A Noble1, Ravishankar Palanivelu2,3

  • 1School of Plant Sciences, University of Arizona, Tucson, AZ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 13, 2020
PubMed
Summary
This summary is machine-generated.

This study introduces a streamlined workflow for characterizing reproductive defects in plant mutants, focusing on Arabidopsis. The method efficiently distinguishes male and female reproductive issues, aiding genetic research.

Keywords:
Aniline blue stainingFertilityMale gametophyte and sporophyteOvulesPistilPollenPollen tubePollen tube–pistil interactionsSeed setTransmission efficiency

More Related Videos

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

4.5K
Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

1.5K

Related Experiment Videos

Last Updated: Dec 18, 2025

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
09:17

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

648
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis
10:32

Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis

Published on: June 10, 2020

4.5K
Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

1.5K

Area of Science:

  • Plant genetics
  • Reproductive biology
  • Developmental biology

Background:

  • Reverse genetics requires detailed genotyping and phenotypic analysis for gene function studies.
  • Characterizing mutants with genes of interest (GOI) in reproductive tissues presents unique challenges due to intertwined sporophytic and gametophytic development.
  • Identifying specific reproductive defects in mutants is crucial for understanding plant reproduction.

Purpose of the Study:

  • To present a streamlined workflow for characterizing reproductive defects in plant mutants.
  • To provide a method for distinguishing between male and female reproductive defects.
  • To adapt these procedures for Arabidopsis and other flowering plants.

Main Methods:

  • Development of a systematic workflow for mutant characterization.
  • Application of various assays to analyze reproductive tissues (sporophytic and gametophytic).
  • Focus on distinguishing defects in male reproductive tissues, with applicability to female defects.

Main Results:

  • The workflow effectively streamlines the characterization of reproductive mutants.
  • It allows for the precise identification of defective reproductive steps.
  • The method is adaptable for both male and female reproductive defect analysis in model plants.

Conclusions:

  • The presented workflow offers an efficient approach to studying reproductive mutants in plants.
  • This method aids in pinpointing specific defects in male and/or female reproductive development.
  • The workflow is valuable for genetic research in flowering plants, including Arabidopsis.