Related Experiment Video
Updated: Dec 18, 2025
Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
Published on: September 27, 2024
Simultaneous imaging of lysosomal and mitochondrial viscosity during mitophagy using molecular rotors with dual-color
Wen Chen1, Junyan Han1, Jiaxin She1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. jianhuijiang@hnu.edu.cn lzhu@hnu.edu.cn.
Abstract:
Two molecular rotors with a single excitation and dual-color emission wavelengths were engineered for simultaneous imaging of lysosomal and mitochondrial viscosity. These two molecular rotors were highly sensitive to viscosity without dependence on pH or polarity, facilitating simultaneous imaging of lysosomal and mitochondrial viscosity during mitophagy in living cells.
