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Related Experiment Videos

Endoplasmin is a reticuloplasmin.

G L Koch1, D R Macer, F B Wooding

  • 1Medical Research Council Laboratory of Molecular Biology, Cambridge, England.

Journal of Cell Science
|July 1, 1988
PubMed
Summary

Endoplasmin is a soluble luminal protein within the endoplasmic reticulum (ER), not an integral membrane protein. Biochemical and immunoelectron microscopy confirm its uniform distribution throughout the ER lumen.

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THE FINE STRUCTURE AND DEVELOPMENT OF THE COMPANION CELL OF THE PHLOEM OF ACER PSEUDOPLATANUS.

The Journal of cell biology·2009

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Localization

Background:

  • Endoplasmin's precise location within the endoplasmic reticulum (ER) has been debated.
  • Understanding protein localization is crucial for elucidating cellular functions.

Purpose of the Study:

  • To definitively determine whether endoplasmin is a luminal or integral membrane protein of the ER.
  • To characterize the biophysical properties of endoplasmin relevant to its localization.

Main Methods:

  • Biochemical analyses, including detergent-free extraction and Triton X-114 phase separation.
  • Immunoelectron microscopy with immunogold labeling on ER-dilated cells.

Main Results:

  • Endoplasmin was extracted in a soluble form without detergents, indicating low hydrophobicity.
  • Triton X-114 phase separation confirmed endoplasmin is not significantly hydrophobic.
  • Immunogold labeling showed uniform distribution within the ER lumen, not membrane-associated.

Conclusions:

  • Endoplasmin is a soluble luminal protein of the endoplasmic reticulum (ER).
  • It is classified as a reticuloplasmin, distinct from integral membrane proteins.
  • These findings clarify endoplasmin's role as a resident ER luminal protein.

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