Chromosomal location of gene governing the trehalose utilization in Escherichia coli K12

Several mutants unable to utilize trehalose were isolated from Escherichia coli, Their genetic analysis led to determine the following gene order on the chromosomal map: pur B-dad R-tre-hem a-trp. Furthermore, the tre gene belongs to the inversion of the trp chromosomal region between E. coli and S. typhimurium.