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Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Updated: Dec 18, 2025

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

Published on: July 20, 2016

Tyrosine Conjugation Methods for Protein Labelling.

Dimitri Alvarez Dorta¹, David Deniaud¹, Mathieu Mével²

¹CNRS, CEISAM UMR, 6230, Université de Nantes, 44000, Nantes, France.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|June 16, 2020

Summary

This summary is machine-generated.

New bioconjugation methods target tyrosines (Tyr) for precise protein labeling, offering alternatives to traditional lysine and cysteine strategies for creating advanced antibody-drug conjugates and probes.

Keywords:

bioconjugation bioorthogonality click chemistry protein modification tyrosine

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Area of Science:

Chemical biology
Bioconjugation techniques

Background:

The need for defined protein conjugates has driven research into novel bioconjugation methods.
Tyrosine (Tyr) residues, with their unique properties, present an attractive target for site-selective protein labeling.
Conventional methods often target lysine and cysteine residues, limiting specific functionalization.

Purpose of the Study:

To review and discuss emerging biorthogonal labeling strategies targeting the tyrosine side chain.
To highlight the advantages of tyrosine-targeting over traditional bioconjugation approaches.
To showcase applications in developing advanced biomolecules.

Main Methods:

Review of biorthogonal labeling strategies.
Functionalization of the tyrosine phenol side chain.
Site-selective protein modification.

Main Results:

Tyrosine targeting enables precise functionalization of proteins.
Successful applications include antibody-drug conjugates, protein probes, and PEGylation.
Innovative methods allow ligand-directed anchoring and affinity capture.

Conclusions:

Tyrosine-targeting bioconjugation offers a powerful alternative to conventional methods.
These techniques are crucial for advancing the design of complex biomolecules.
Further development promises enhanced capabilities in protein engineering and drug delivery.