Related Experiment Videos
Specific effects of nerve growth factor on the differentiation pattern of mouse embryonic stem cells in vitro
A M Wobus1, R Grosse, J Schöneich
1Zentralinstitut für Genetik und Kulturpflanzenforschung der AdW der DDR, Gatersleben.
Abstract:
The effect of nerve growth factor (NGF) on the differentiation pattern of pluripotent undifferentiated mouse embryonic stem cells (ESC) was studied. Morphological analysis of differentiating stem cells and histochemical silver-staining analysis of embryonic carcinoma (P19S1801A1) and embryonic stem cells (ESC-BLC 6) permitted the demonstration of nerve cell differentiation. NGF treatment resulted in an accelerated appearance of neuron-like cells in differentiating embryoid bodies of ESC line BLC 6. By use of the silver-staining test NGF dependent induction of nerve cell lineage was demonstrated. The presented embryonic stem cell model allows to study the time course of differentiation and induction of a special cell lineage of undifferentiated embryonic stem cells by growth factors.