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Updated: Dec 18, 2025
Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
Published on: June 16, 2023
Long Non-Coding RNA 691 Regulated PTEN/PI3K/AKT Signaling Pathway in Osteosarcoma Through miRNA-9-5p
Pengju Yao1, Yangming Ni1, Changlu Liu2
1Department of Joint Surgery, Jiaozuo People's Hospital, Henan Province, People's Republic of China.
Background:
Large amounts of researches indicate that non-coding RNAs play a crucial role in many malignancies. However, the potential mechanisms of non-coding RNAs involved in osteosarcoma tumorigenesis remain elusive.
Materials And Methods:
The expression of long non-protein coding RNA 691 (lncRNA 691) in cell lines and paired osteosarcoma tissues was compared by qRT-PCR assay. Then, we explored the tumor suppressor function of lncRNA 691 with MTS and colony formation assay. Flow cytometry results showed lncRNA 691 can enhance cell apoptosis. Then, we predicted and verified the negative regulation relationship with miRNA and the miRNA's target gene. Lastly, we revealed the tumorigenesis function of lncRNA-691/miRNA/target gene axis in osteosarcoma.
Results:
In our study, we disclosed that lncRNA 691 had low expression levels in osteosarcoma cell lines and tissues. Overexpression of lncRNA 691 could suppress the cell proliferation and induce cell apoptosis in MG-63 cell line. Then, bioinformatics analyses were performed and miR-9-5p was found to negatively regulate the lncRNA 691 expression and promote the osteosarcoma tumorigenesis in vitro. PTEN was predicted as the target gene of miR-9-5p. Luciferase reporter assay and RIP assay demonstrated the regulatory network of lncRNA 691/miR-9-5p/PTEN. We revealed that PTEN was downregulated by the overexpression of miR-9-5p and upregulated by the overexpression of lncRNA 691. At last, the apoptosis-associated protein of the lncRNA 691/miR-9-5p/PTEN/PI3K/AKT was further demonstrated.
Conclusion:
LncRNA 691/miR-9-5p could regulate the tumorigenesis by regulating the PTEN/PI3K/AKT signal pathway in osteosarcoma.
Insights
Long non-coding RNA 691 (lncRNA 691) acts as a tumor suppressor in osteosarcoma by inhibiting cell proliferation and promoting apoptosis. It regulates the miR-9-5p/PTEN axis, impacting the PI3K/AKT pathway in osteosarcoma tumorigenesis.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- Non-coding RNAs are implicated in various cancers.
- The specific role of non-coding RNAs in osteosarcoma development is not fully understood.
Purpose of the Study:
- To investigate the function of long non-protein coding RNA 691 (lncRNA 691) in osteosarcoma.
- To elucidate the molecular mechanisms underlying lncRNA 691's role in osteosarcoma tumorigenesis.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) to assess lncRNA 691 expression.
- Cell proliferation (MTS, colony formation) and apoptosis assays (flow cytometry).
- Bioinformatic analysis, luciferase reporter assays, and RIP assays to determine regulatory interactions.
Main Results:
- lncRNA 691 expression is low in osteosarcoma tissues and cell lines.
- Overexpression of lncRNA 691 suppresses proliferation and induces apoptosis.
- lncRNA 691 negatively regulates miR-9-5p, which targets PTEN, affecting the PI3K/AKT pathway.
Conclusions:
- lncRNA 691 functions as a tumor suppressor in osteosarcoma.
- The lncRNA 691/miR-9-5p/PTEN axis regulates osteosarcoma tumorigenesis via the PI3K/AKT signaling pathway.
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