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Updated: Dec 18, 2025

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Published on: September 11, 2022
Electrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe
Shirin Khaliliazar1, Liangqi Ouyang1, Andrew Piper1
1Department of Fibre and Polymer Technology; School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Tekninkringen 56-58, SE-100 44 Stockholm, Sweden.
Abstract:
Nucleic acid tests integrated into digital point-of-care (POC) diagnostic systems have great potential for the future of health care. However, current methods of DNA amplification and detection require bulky and expensive equipment, many steps, and long process times, which complicate their integration into POC devices. We have combined an isothermal DNA amplification method, recombinase polymerase amplification, with an electrochemical stem-loop (S-L) probe DNA detection technique. By combining these methods, we have created a system that is able to specifically amplify and detect as few as 10 copies/μL Staphylococcus epidermidis DNA with a total time to result of 70-75 min.
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