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Related Concept Videos

Centrifugation01:05

Centrifugation

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Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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Related Experiment Video

Updated: Dec 18, 2025

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
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Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

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Platelet removal by single-step centrifugation.

Linda G Rikkert1,2,3, Frank A W Coumans3,4, Chi M Hau2,3

  • 1Medical Cell BioPhysics, University of Twente, Enschede, The Netherlands.

Platelets
|June 20, 2020
PubMed
Summary
This summary is machine-generated.

A new single-step centrifugation protocol effectively removes platelets from plasma for extracellular vesicle (EV) research. This faster, easier method yields more plasma while maintaining EV recovery, improving sample quality for biomarker studies.

Keywords:
Bloodblood plateletscentrifugationextracellular vesiclesflow cytometry

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Clinical Diagnostics

Background:

  • Extracellular vesicles (EVs) in plasma are crucial biomarkers.
  • Current methods for plasma preparation, like two-step centrifugation, are laborious and operator-dependent.
  • High-quality plasma free of cellular contaminants, especially platelets, is essential for reliable EV research.

Purpose of the Study:

  • To develop a simple, rapid, and clinically applicable centrifugation protocol for platelet-free plasma.
  • To optimize plasma collection for extracellular vesicle (EV) research.
  • To enhance the quality and yield of plasma samples for biomarker discovery.

Main Methods:

  • Comparison of a novel single-step centrifugation protocol (5,000 g for 20 minutes) against the standard two-step protocol.
  • Computational prediction and experimental validation of platelet removal efficiency.
  • Flow cytometry analysis to quantify residual platelets and platelet-derived EVs (CD61+).

Main Results:

  • The single-step protocol is less laborious and approximately 10 minutes faster than the two-step method.
  • Platelet removal efficacy of the single-step protocol is comparable to the two-step protocol.
  • The single-step protocol achieves a ~10% higher plasma yield with similar recovery of platelet-derived EVs.

Conclusions:

  • The developed single-step centrifugation protocol is an efficient and practical method for preparing platelet-free plasma.
  • This protocol improves plasma yield and reduces processing time, making it suitable for clinical applications.
  • Recommended for future research on plasma EVs and other biomarkers, enhancing sample quality and reproducibility.