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[Establishment of Acquired Aplastic Anemia SD Rat Model]
Ping-Ping Wang1, Jia-Ni Li1, Xin-Long Song2
1Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.
Objective:
To establish a stable and rapidly rat model acquired aplastic anemia.
Methods:
The SD rats were exposed to 137Csγ-ray at 3.5 and 4.0 Gy ( 91 cGy/min), and intraperitoneally injected with CTX at 35 mg/( kg·d) and CHL at 45 mg/( kg·d) in d 4, 6 and 8 after irradiation; the WBC, platelet and reticulocyte counts in peripheral blood, the smears and nucleated cells counts of bone marrow were observed.
Results:
The levels of peripheral blood 3-lineage cells of SD rats treated with 137Csγ-irradiation combined with cyclophosphamide and chloramphenicol were significantly reduced, among which white blood cells, platelets and reticulocytes decreased rapidly, and the number of bone marrow nucleated cells decreased significantly; bone marrow pathological sections showed severe reduction of hematopoietic cells, and the non-hematopoietic cells such as fat cells increased, and a serve or lightly reduction of bone marrow cells were found.
Conclusion:
The rat model established by 137Csγ-ray irradiation combined with cyclophosphamide and chloramphenicol meets the clinical characteristics of aplastic anemia, and this study provides a stable rat model for the study of new therapeutic drugs for acquired aplastic anemia.
