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Updated: Dec 18, 2025

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
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Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

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Abemaciclib induces apoptosis in cardiomyocytes by activating the Hippo signaling pathway

Yajie Zhou1,2, Yanfei Li3, Junwei Shen4

  • 1Clinical Medical College, Guizhou Medical University, Guiyang 550004, China.

Acta Biochimica Et Biophysica Sinica
|June 20, 2020
PubMed

Insights

Abemaciclib, a new breast cancer drug, may harm heart cells by triggering apoptosis and inhibiting proliferation via the Hippo pathway. This research reveals the molecular mechanism behind potential cardiac side effects.

Area of Science:

  • Cardiology
  • Oncology
  • Molecular Biology

Background:

  • Abemaciclib is an FDA-approved cyclin-dependent kinase 4/6 inhibitor for advanced breast cancer.
  • Potential adverse effects on cardiomyocytes are not well understood.

Purpose of the Study:

  • To investigate the effects of abemaciclib on human cardiomyocyte cells.
  • To elucidate the molecular mechanisms underlying abemaciclib-induced cardiotoxicity.

Main Methods:

  • Cell counting kit-8 assay
  • Western blot analysis
  • Flow cytometry
  • Immunostaining
  • Quantitative polymerase chain reaction (qPCR) on AC16 human cardiomyocyte cells.

Main Results:

  • Abemaciclib significantly inhibited viability and proliferation of AC16 cells.
  • Abemaciclib induced apoptosis in AC16 cells.
  • Activation of the Hippo signaling pathway was observed.

Conclusions:

  • Abemaciclib induces cardiomyocyte apoptosis and inhibits proliferation through Hippo pathway activation.
  • This study provides insights into the molecular basis of abemaciclib's cardiac side effects.
  • Identifies potential therapeutic targets for managing cardiotoxicity.

Keywords:
CDK4/6 inhibitorHippo signaling pathwayabemaciclibcardiomyocytes

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