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Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
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Virus-Induced Gene Silencing to Investigate Alkaloid Biosynthesis in Opium Poppy
Rongji Chen1,2, Xue Chen1,2, Jillian M Hagel1,2
1Epimeron Inc., Calgary, AB, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|June 20, 2020
Abstract:
Virus-induced gene silencing (VIGS) enables the targeted silencing of genes in opium poppy (Papaver somniferum) and has been used extensively to determine or support the physiological functions of benzylisoquinoline alkaloid biosynthetic enzymes. Here we describe detailed protocols involved in the application of VIGS to investigate BIA metabolism in opium poppy.
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