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Updated: Dec 17, 2025
Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
Published on: October 3, 2014
Tissue engineering using a combined cell sheet technology and scaffolding approach
Irina M Zurina1, Viktoria S Presniakova2, Denis V Butnaru3
1Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 119991 8-2 Trubetskaya St., Moscow, Russia; FSBSI Institute of General Pathology and Pathophysiology, 125315, 8 Baltiyskaya St., Moscow, Russia; FSBEI FPE "Russian Medical Academy of Continuous Professional Education" of the Ministry of Healthcare of Russia, 125993, 2/1-1 Barrikadnaya St., Moscow, Russia.
Combining cell sheets with scaffolds enhances tissue engineering. This review analyzes the advantages, limitations, and applications of this integrated approach for regenerating bone, cartilage, and soft tissues.
Area of Science:
- Regenerative Medicine
- Tissue Engineering
- Biomaterials Science
Background:
- Cell sheet technology is a popular tissue engineering technique.
- A growing trend involves combining cell sheets with supporting scaffolds.
- This integrated approach offers potential for complex tissue regeneration.
Purpose of the Study:
- To review the intersection of cell sheet technology and scaffolds in tissue engineering.
- To analyze advantages, limitations, and practical considerations of combined approaches.
- To summarize in vivo studies on tissue regeneration using combined strategies.
Main Methods:
- Literature review focusing on the last five years.
- Analysis of studies combining cell sheets and scaffolds.
- Categorization of applications by tissue type (bone, cartilage, soft tissues).
Main Results:
- Combined cell sheet-scaffold technology presents unique advantages and limitations.
- Significant applications are found in bone regeneration.
- The approach is also effective for other connective and soft tissues.
Conclusions:
- Integrated cell sheet and scaffold strategies are crucial for advanced tissue engineering.
- This combined method shows promise for diverse regenerative medicine applications.
- Further research can optimize designs for clinical translation.
