Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

<i>Manilkara zapota</i>: From Phytochemistry to Therapeutics, and Relevance to Food Industries.

Foods (Basel, Switzerland)·2026

Same author

Phytochemical Fingerprints by GC-MS, Antidiabetic and Multiorgan Protective Effects of Cucumis sativus Peel: An Integrated In Vitro, In Vivo, and In Silico Study.

Chemistry & biodiversity·2026

Same author

Portable hepatitis C virus RNA extraction and stabilization using low-cost lab-on-a-chip style components with shelf stable reagents.

Sensors and actuators. B, Chemical·2026

Same author

Investigating the phytotherapeutic efficacy of <i>Acacia nilotica</i> pod ethanolic extract in modulating lipid profile, oxidative stress and inflammation in diet-induced hypercholesterolemia: <i>in vivo</i> and <i>in silico</i> insights.

Frontiers in pharmacology·2026

Same author

Qualitative and quantitative assessment of <i>ex vivo</i> human brain tumors using quantitative oblique back-illumination microscopy (qOBM).

Biomedical optics express·2026

Same author

Sea Buckthorn (<i>Hippophae rhamnoides</i> L.): Nutritional Significance, Phytochemistry, Molecular Mechanisms, Therapeutic Potential, and Emerging Applications in Food Systems.

Foods (Basel, Switzerland)·2026

Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 17, 2025

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

Published on: April 28, 2022

Label-free hematology analysis using deep-ultraviolet microscopy.

Ashkan Ojaghi¹, Gabriel Carrazana¹, Christina Caruso^2,3,4

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332.

Proceedings of the National Academy of Sciences of the United States of America

|June 21, 2020

Summary

This summary is machine-generated.

This study introduces a novel label-free deep-ultraviolet microscopy assay for rapid hematological analysis. The method provides quantitative blood cell counts and morphology without complex procedures, simplifying complete blood counts (CBCs).

Keywords:

deep-UV microscopy hematology analysis label-free cell classification molecular imaging point-of-care diagnosis

More Related Videos

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

Related Experiment Videos

Last Updated: Dec 17, 2025

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

Published on: April 28, 2022

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Lensless On-chip Imaging of Cells Provides a New Tool for High-throughput Cell-Biology and Medical Diagnostics

Published on: December 14, 2009

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

Area of Science:

Biomedical Imaging
Hematology
Microscopy

Background:

Traditional complete blood count (CBC) and microscopy require complex equipment, reagents, and trained personnel.
Current methods for blood analysis are laborious and time-consuming, limiting accessibility in low-resource settings.

Purpose of the Study:

To develop a simplified, rapid, and quantitative hematological assay.
To enable label-free molecular imaging for improved blood condition diagnosis and monitoring.

Main Methods:

Utilized deep-ultraviolet microscopy for label-free molecular imaging of blood cells.
Developed a pseudocolorization scheme to mimic conventional staining protocols.
Analyzed tens of thousands of live cells in minutes without sample preparation.

Main Results:

Achieved quantitative five-part white blood cell differential.
Provided quantitative red blood cell and hemoglobin characterization.
Enabled clear platelet identification and detailed subcellular morphology analysis.

Conclusions:

The label-free deep-ultraviolet microscopy assay significantly simplifies and improves CBC and blood smear analysis.
This technology has potential for developing low-cost, point-of-care hematological analyzers for diverse settings.