Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The Sarcomere

The Sarcomere

A sarcomere is a microscopic segment repeating in a myofibril. The sarcomere fundamentally consists of two main myofilaments: thick filaments called myosin and thin filaments called actin. These filaments interact by sliding past each other in response to stimulus. In addition to myosin and actin, several other proteins, such as tropomyosin, troponin, titin, nebulin, myomesin, α-actinin, and dystrophin, play crucial roles in regulating, structuring, and functioning of the sarcomere.
Each...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The DRoPH score: development of a tool for predicting reduction difficulty in proximal humerus fracture osteosynthesis.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2026

Same author

Sequential changes in calcium transients during M phase regulate cardiomyocyte proliferation.

The Journal of cell biology·2026

Same author

High-Affinity Human Germline and Mutated Autoantibodies Commonly Cross-React to Self-Antigens in 5 Subgroups of Autoimmune Encephalitis.

Neurology(R) neuroimmunology & neuroinflammation·2026

Same author

Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy.

Signal transduction and targeted therapy·2026

Same author

Standardized processing of explanted hearts after stereotactic arrhythmia radiotherapy (Bio-STAR): a STOPSTORM framework and first human findings.

European heart journal open·2026

Same author

Septin multimer autoantibodies in severe motor neuropathy mimicking lower motor neuron disease.

Brain : a journal of neurology·2026

Search research articles

Related Experiment Video

Updated: Dec 17, 2025

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

Published on: June 22, 2020

Deconstructing sarcomeric structure-function relations in titin-BioID knock-in mice.

Franziska Rudolph¹, Claudia Fink¹, Judith Hüttemeister¹

¹Neuromuscular and Cardiovascular Cell Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Robert Rössle Strasse, 1013125, Berlin, Germany.

Nature Communications

|June 21, 2020

Summary

This study uses proximity proteomics in mice to map sarcomere protein networks, revealing how muscle develops and shifts metabolism. Findings enhance understanding of muscle cell function and disease.

More Related Videos

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

Published on: March 3, 2021

Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development

Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development

Published on: July 1, 2020

Related Experiment Videos

Last Updated: Dec 17, 2025

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

Published on: June 22, 2020

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

Published on: March 3, 2021

Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development

Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development

Published on: July 1, 2020

Area of Science:

Biochemistry
Molecular Biology
Physiology

Background:

Proximity proteomics is valuable for studying protein complexes in vitro but limited for in vivo studies.
Understanding in vivo protein interactions is crucial for development and disease research.

Purpose of the Study:

To develop an in vivo proximity proteomics approach to study sarcomere protein networks.
To identify protein interactions connecting the sarcomere to signal transduction and metabolism in developing and adult muscle.

Main Methods:

Generated a knock-in mouse model with biotin ligase (BioID) fused to titin at the Z-disc.
Performed proximity-dependent biotinylation and proteomic analysis of cardiac and quadriceps muscle from neonatal to adult stages.

Main Results:

Cataloged the sarcomeric proteome, detailing changes in protein networks during muscle development and metabolic shifts.
Mapped biotinylation sites to refine understanding of myofilament dynamics and Z-disc function.
Provided insights into perinatal signaling, protein homeostasis, and adult energy metabolism in striated muscle.

Conclusions:

The developed BioID mouse model is a powerful tool for in vivo sarcomere proteome analysis.
This approach advances the study of molecular machines and signaling hubs in muscle physiology and disease.
Future applications include studying pathological conditions using BioID fusion proteins in disease models.