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Chitosan derivatives and their based nanoparticles: ultrasonic approach to the synthesis, antimicrobial and
Andreii S Kritchenkov1, Natallia V Zhaliazniak2, Anton R Egorov3
1Peoples' Friendship University of Russia (RUDN University), Miklukho-Maklaya St. 6, Moscow, 117198, Russian Federation; Saint Petersburg National Research University of Information Technologies, Mechanics, and Optics, Kronverkskii pr. 49, 197101 St. Petersburg, Russian Federation; Institute of Technical Acoustics NAS of Belarus, Ludnikova Prosp. 13, Vitebsk, 210009, Republic of Belarus.
Abstract:
In the present work, we demonstrate that alkylation of chitosan by alkyl halides, aza-Michael reaction with chitosan, and AdN-E reaction of chitosan with aldehydes can be efficiently mediated by ultrasound. An optimization of ultrasonic irradiation parameters allowed us to (i) accelerate the rate of the reactions dramatically, (ii) achieve high selectivity, and (iii) preserve integrity of the polysaccharide backbone avoiding its depolymerization. We evaluated antibacterial/antifungal and transfection activity of 8 different derivatives of chitosan and their based nanoparticles in vitro. Moreover, we studied antibacterial activity of the most efficient polymer and their based nanoparticles in vivo. The tested polymer proved to be superior to reference commercial antibiotics ampicillin and gentamicin.
