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Updated: Dec 17, 2025

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
Published on: April 5, 2019
The challenge of separating and determining biologically active electrostatically stabilized silanates using the
Joanna Jabłońska1, Mariusz Kluska1, Nikolai Erchak2
1Faculty of Exact and Natural Sciences, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland.
Abstract:
We present the results of research on the optimal conditions for the separation and determination of newly obtained hypercoordinated compounds, which belong to the group of electrostatically stabilized silanates. The research involved five stationary and four mobile phases. The best selectivity was obtained using the graphite phase and the mobile phase consisting of acetonitrile/water (80/20). The maximum selectivity of the determined electrostatically stabilized silanates was 1.13 and 1.06 for (1), (2), (3); 1.10 and 1.15 for (4), (5), (6); and 1.12 and 1.15 for (7), (8), (9). The octadecyl phase (which is recommended as standard) did not yield satisfactory results.
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