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[3H]-forskolin binding sites in rat submandibular salivary glands
1Louisiana State University Medical Center, Department of Biochemistry and Molecular Biology, New Orleans 70119.
Abstract:
Specific [3H]-forskolin binding was linear as a function of the membrane protein concentration and reached steady-state by 30-40 min of incubation at 37 degrees C. Such binding was saturable and the bound radioligand could be readily dissociated by an excess of unlabelled forskolin. There was only one type of binding site, with a dissociation constant (Kd) of 0.16 microM. The concentration of the binding sites (Bmax) was 0.73 pmol/mg protein. There was no significant difference in the [3H]-forskolin binding characteristics in membranes of rats fed for 6 weeks on diets containing 10% corn oil, 8% coconut oil + 2% corn oil, or 10% menhaden fish oil.