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miR-151a enhances Slug dependent angiogenesis.

Douglas Jury1, Iben Daugaard1,2,3, Katie J Sanders1

  • 1Department of Molecular Biology and Biochemistry, School of Biological Sciences, University of California, Irvine, CA 92697, USA.

Oncotarget
|June 25, 2020
PubMed
Summary

MicroRNA-151a promotes tumor growth and angiogenesis in lung cancer. Inhibiting miR-151a enhances endothelial cell barrier function, suggesting dual roles in cancer progression and vascular health.

Keywords:
Slugangiogenesisendothelial cellmiR-151atumor microenvironment

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Area of Science:

  • Molecular Biology
  • Oncology
  • Vascular Biology

Background:

  • MicroRNAs (miRs) are key regulators of gene expression impacting tissue homeostasis and disease.
  • Aberrant angiogenesis is implicated in pathological conditions like cancer and ischemia.
  • miR-151a was previously identified as an onco-miR promoting non-small cell lung cancer (NSCLC) growth.

Purpose of the Study:

  • To investigate the role of miR-151a in angiogenesis and endothelial cell function.
  • To elucidate the mechanisms by which miR-151a influences tumor-associated vascularization.
  • To understand the multifaceted roles of miR-151a in lung tissue.

Main Methods:

  • Assessing endothelial cell contacts and barrier properties.
  • Evaluating endothelial cell motility and angiogenesis.
  • Utilizing 3D vascularized tumor spheroid assays.
  • Confirming miR-151a expression in lung tissues.

Main Results:

  • Anti-miR-151a promoted endothelial cell contacts and barrier properties.
  • Induced miR-151a expression enhanced endothelial cell motility and angiogenesis, dependent on Slug levels.
  • miR-151a overexpression increased tumor-associated angiogenesis in vitro.
  • miR-151a was detected in the vasculature of normal and NSCLC lung tissues.

Conclusions:

  • miR-151a exhibits dual roles, promoting NSCLC growth and angiogenesis while its inhibition strengthens endothelial barriers.
  • miR-151a regulates angiogenesis through Slug-dependent mechanisms.
  • miR-151a plays complex, cell-type-specific roles in lung physiology and pathology, including cancer.