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Protective effect of seabuckthorn berry juice against acrylamide-induced oxidative damage in rats
Sijia Zhao1,2, Hongyang Sun3, Qingbo Liu1,2
1College of Food Science, Northeast Agricultural University, Harbin, 150030, China.
Abstract:
Acrylamide (AA), classified as a probable carcinogen, can be neurotoxic, genotoxic, and can damage DNA. This study explored the ability of seabuckthorn berries juice (SBJ) to alleviate AA-induced toxic injury in rats. Twenty-four adult male Sprague-Dawley (SD) rats were randomly divided into four groups: control group, AA group (40 mg/kg), AA + SBJ (40 mg/kg AA and 5 mL/kg SBJ), and AA + vitamin C (VC) group (positive control group, 40 mg/kg AA and 100 mg/kg VC). At the end of the experiment, rats in AA group showed a marked decrease in the rate of weight gain, hind extremity abduction, and ataxia. Obvious anomalies were seen in plasma biochemical parameters (P < 0.05), and different degrees of injury were observed upon histological examination of five tissues (hippocampus, cerebellum, liver, small intestine, and kidney). Compared to the control group, levels of superoxide dismutase, catalase, and glutathione were significantly decreased, while malondialdehyde was elevated (P < 0.05). SBJ treatment reduced the abnormal of behavior, hematological index, antioxidant enzyme, and tissue damage caused by AA in rats. PRACTICAL APPLICATION: Seabuckthorn berries are wild berries rich in vitamin C and polyphenols, which have good antioxidant properties. In this experiment, SBJ has a significant alleviating effect on AA-induced oxidative damage in rats. Therefore, we speculate that SBJ may relieve the oxidative damage caused by diet or other forms of AA exposure in the general population. At the same time, this experiment also provides new ideas for alleviating AA-induced in vivo toxicity.
Insights
Seabuckthorn berry juice (SBJ) effectively counteracts acrylamide (AA)-induced toxicity in rats by reducing oxidative stress and tissue damage. This suggests SBJ may protect against AA exposure in humans.
Area of Science:
- Toxicology
- Nutritional Science
- Biochemistry
Background:
- Acrylamide (AA) is a probable carcinogen with known neurotoxic and genotoxic effects.
- Oxidative stress plays a significant role in AA-induced cellular and tissue damage.
- Natural antioxidants are being investigated for their potential to mitigate toxicant-induced injuries.
Purpose of the Study:
- To investigate the protective effects of seabuckthorn berry juice (SBJ) against acrylamide (AA)-induced toxicity in Sprague-Dawley rats.
- To evaluate SBJ's impact on AA-induced behavioral changes, biochemical parameters, oxidative stress markers, and tissue histology.
Main Methods:
- Twenty-four male Sprague-Dawley rats were divided into four groups: control, AA-exposed, AA + SBJ, and AA + vitamin C (positive control).
- AA was administered at 40 mg/kg, SBJ at 5 mL/kg, and vitamin C at 100 mg/kg.
- Evaluations included weight gain, behavioral observations, plasma biochemical analysis, antioxidant enzyme levels (superoxide dismutase, catalase, glutathione), malondialdehyde levels, and histological examination of key tissues (hippocampus, cerebellum, liver, small intestine, kidney).
Main Results:
- Acrylamide exposure led to decreased weight gain, hind limb ataxia, abnormal plasma biochemical parameters, and histological damage in multiple organs.
- AA-exposed rats exhibited significantly reduced antioxidant enzyme activity and elevated malondialdehyde levels, indicating oxidative stress.
- SBJ treatment notably ameliorated behavioral abnormalities, improved hematological indices, restored antioxidant enzyme levels, and reduced tissue damage compared to the AA group.
Conclusions:
- Seabuckthorn berry juice demonstrates significant protective effects against acrylamide-induced oxidative damage and toxicity in rats.
- SBJ's antioxidant properties, attributed to its rich vitamin C and polyphenol content, are key to its mitigating effects.
- SBJ shows potential as a dietary intervention to alleviate oxidative stress from acrylamide exposure in the general population.
