Investigating the effect of antiseptic solution on the release of interleukin-6 and transforming growth factor beta 1...

This in vitro study evaluated the effect of different antiseptics and different concentrations thereof in a model of wound healing using human gingival fibroblasts. The fibroblasts were rinsed with four different antiseptic solutions: sodium hypochlorite (HYP), hydrogen peroxide (H₂O₂), chlorhexidine digluconate (CHX), and benzalkonium chloride (BC). The effect on the release of interleukin-6 (IL-6) and transforming growth factor beta 1 (TGF-β1) was investigated using enzyme-linked immunosorbent assays (ELISAs). In addition, the effects of the antiseptics on wound healing at 1, 12, 24, and 48 h were assessed through a wound healing assay. The viability of the fibroblasts rinsed with antiseptics was investigated with respect to the concentrations inhibiting cell growth by 50% (IC₅₀), 25% (IC₂₅), and ≤2% (IC₂). A statistically significant increased release of IL-6 was obtained with BC IC₂₅ and IC₂ after 12, 24, and 48 h (P < 0.01). For TGF-β1, no significant release was found for CHX IC₂ after 24 and 48 h or for IC₅₀ and IC₂₅ after 12 h. There was no significant effect on wound healing capacity for CHX or for BC IC₂₅ and IC₂. This study demonstrated that antiseptic rinses of human gingival fibroblasts alter the release of IL-6 and TGF-β1 and impact wound healing capacity, with both BC and CHX conferring neutral effects.