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Human apolipoprotein L1 interferes with mitochondrial function in Saccharomyces cerevisiae
Mounia Chidiac1, Jalil Daher2, Mélanie Boeckstaens3
1Laboratory of Molecular Parasitology, Laboratory of Gene Molecular Biology, IBMM, Université Libre de Bruxelles, 6041 Gosselies, Belgium.
Molecular Medicine Reports
|June 26, 2020
Summary
The human apolipoprotein L1 (APOL1) protein disrupts mitochondrial function and morphology in yeast. This suggests APOL1 may regulate mitochondrial processes in vivo.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- The vertebrate apolipoprotein L (APOL) family's function is largely unknown.
- The conserved BH3 domain suggests roles in programmed cell death or mitochondrial processes.
Purpose of the Study:
- To determine the molecular function of human APOL1.
- To investigate APOL1's role in cellular processes and mitochondrial function.
Main Methods:
- Expressed human APOL1 in Saccharomyces cerevisiae.
- Assessed cell proliferation on fermentable and non-fermentable carbon sources.
- Utilized Western blotting and GFP tagging for protein localization and mitochondrial morphology analysis.
Main Results:
- APOL1 inhibited yeast proliferation on glycerol but not galactose.
- APOL1 localized to mitochondria and impaired mitochondrial function (respiratory index, membrane potential).
- APOL1 induced abnormal mitochondrial and lysosomal morphology.
Conclusions:
- APOL1 interferes with mitochondrial function and morphology.
- APOL1 may act as a physiological regulator of mitochondrial function.
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