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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

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Application of Various Delivery Methods for CRISPR/dCas9.

Zhixi Liu^1,2,3, Zhi Liao⁴, Yan Chen¹

¹Department of Clinical Pharmacy, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, No. 55, Section 4, South Renmin Road, Chengdu, 610041, Sichuan, China.

Molecular Biotechnology

|June 26, 2020

Summary

CRISPR gene editing is a powerful tool in life sciences, driving advances in research and medicine. This paper reviews CRISPR/dCas9 delivery methods, a key challenge for its effective application.

Keywords:

CRISPR Delivery dCas9

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Area of Science:

Molecular Biology
Biotechnology
Genetics

Background:

CRISPR technology offers efficient and versatile gene editing capabilities.
Its applications span basic research, agriculture, medicine, and clinical treatments.
Effective delivery of CRISPR/dCas9 systems to target cells remains a significant challenge.

Purpose of the Study:

To introduce the CRISPR/dCas9 system.
To review common delivery methods for CRISPR/dCas9.
To discuss the characteristics of various delivery techniques.

Main Methods:

Literature review of CRISPR/dCas9 delivery strategies.
Analysis of different delivery system characteristics.
Comparison of existing delivery approaches.

Main Results:

The CRISPR/dCas9 system is detailed.
Various delivery methods, including viral and non-viral vectors, are presented.
Key features and limitations of each delivery method are outlined.

Conclusions:

Understanding CRISPR/dCas9 delivery is crucial for therapeutic and research applications.
The choice of delivery method impacts CRISPR system efficiency and specificity.
Further research is needed to optimize delivery for widespread clinical use.