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Related Experiment Video

Updated: Dec 17, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
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P73 C-terminus is dispensable for multiciliogenesis.

Niall Buckley1, Emanuele Panatta1, Nobuhiro Morone1

  • 1Medical Research Council, Toxicology Unit, Department of Pathology, Cambridge University , Cambridge, UK.

Cell Cycle (Georgetown, Tex.)
|June 26, 2020
PubMed
Summary

The p73 protein

Keywords:
P53 familyciliogenesisdevelopment

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • The p53 family transcriptional factor p73 is crucial for development.
  • p73 knockout mice exhibit neurodevelopmental defects, inflammation, and infertility.
  • The full-length N-terminal isoform TAp73 regulates multiciliogenesis.

Purpose of the Study:

  • Investigate the in vivo role of p73 C-terminal isoforms using a novel mouse model.
  • Determine if p73 C-terminal isoforms are essential for multiciliogenesis.

Main Methods:

  • Generated Trp73Δ13/Δ13 mice with an exon 13 deletion.
  • Analyzed TAp73 expression patterns in wild-type and mutant mice.
  • Examined ciliated epithelial lining in airways and brain ependyma.

Main Results:

  • Trp73Δ13/Δ13 mice exhibit an alpha to beta isoform switch without altering TAp73 expression.
  • Airway ciliated epithelium remains unaffected, suggesting p73β compensates for p73α.
  • Brain ependyma shows no TAp73 expression, indicating compensatory mechanisms.

Conclusions:

  • p73 C-terminal isoforms are dispensable for the multiciliogenesis program.
  • Alternative splicing of p73 may have tissue-specific functional effects.