Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nucleic acids

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Endothelial MHC expression is required to initiate T cell-mediated rejection of 3D-printed skin grafts.

JCI insight·2026

Same author

Towards clinical translation of nanomedicines: Formulation scale-up and model systems.

Advanced drug delivery reviews·2026

Same author

Advances in peptide nucleic acid for targeting RNA and genomic DNA.

Cell reports. Physical science·2026

Same author

Engineered cargo-free nanoparticle decoys for phagocytic modulation of macrophages.

Journal of controlled release : official journal of the Controlled Release Society·2026

Same author

Prenatal Intraportal Delivery of Polymeric Nanoparticles to Fetal Rhesus Monkeys (<i>Macaca mulatta</i>).

Human gene therapy·2026

Same author

Endothelial cell MHC molecules are necessary and sufficient to reject 3D-printed human skin grafts in an advanced human immune system mouse.

bioRxiv : the preprint server for biology·2025

Same journal

Targeted delivery of proteolysis-targeting chimeras (PROTAC) and molecular glue degraders (MGD).

Advanced drug delivery reviews·2026

Same journal

Lysosome-targeting degrader delivery system: from formulation design to biomedical applications.

Advanced drug delivery reviews·2026

Same journal

Anti-PEG antibodies in nanomedicine: Mechanisms, risks, and opportunities.

Advanced drug delivery reviews·2026

Same journal

Optimizing macrophage-targeted intracellular delivery systems for safe and effective immunotherapies.

Advanced drug delivery reviews·2026

Same journal

Light-controlled CRISPR-dCas9 epigenome editing: advanced drug-delivery strategies and oncology applications.

Advanced drug delivery reviews·2026

Same journal

Human organ-on-a-chip technology as a catalyst for drug discovery.

Advanced drug delivery reviews·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 23, 2025

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Polymeric vehicles for nucleic acid delivery.

Alexandra S Piotrowski-Daspit¹, Amy C Kauffman², Laura G Bracaglia¹

¹Department of Biomedical Engineering, Yale University, New Haven, CT 06511, United States of America.

Advanced Drug Delivery Reviews

|June 26, 2020

Summary

This summary is machine-generated.

Polymeric vehicles offer versatile gene delivery, protecting nucleic acids and enhancing cellular uptake. Advances in polymer design and preclinical models promise expanded therapeutic applications for gene therapy.

Keywords:

Biocompatible Biodegradable Gene delivery Gene editing In vivo Nanocarriers Nanoparticle Polymeric vehicle Polyplex Sustained release

More Related Videos

Assembly and Characterization of Polyelectrolyte Complex Micelles

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Related Experiment Videos

Last Updated: Nov 23, 2025

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Assembly and Characterization of Polyelectrolyte Complex Micelles

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Area of Science:

Biomaterials Science
Gene Therapy
Nanotechnology

Background:

Polymeric vehicles are crucial for therapeutic gene delivery, protecting genetic material from degradation and facilitating intracellular transport.
The synthesis of polymers allows for diverse molecular designs and vehicle formulations, enabling manipulation of key delivery properties.
Current polymer-based gene delivery strategies show promise in preclinical and clinical settings.

Purpose of the Study:

To review current approaches and strategies for utilizing polymeric vehicles in gene delivery.
To highlight the versatility and adaptability of polymer design for therapeutic gene delivery applications.
To discuss the potential for expanded impact of polymer-based gene therapy.

Main Methods:

Review of existing literature on polymer synthesis and gene delivery vehicles.
Analysis of preclinical and clinical applications of polymeric gene delivery systems.
Discussion of strategies for enhancing polymer-vehicle performance and specificity.

Main Results:

Polymeric vehicles effectively protect nucleic acid cargo from degradation and clearance in vivo.
Tailored polymer properties enable enhanced cargo association, cellular uptake, and endosomal escape.
Significant therapeutic endpoints have been achieved with polymer vehicles in vitro and in animal models.

Conclusions:

Polymer-based gene delivery is a versatile and effective therapeutic strategy.
Improvements in preclinical models, target specificity, and scalable synthesis will drive future advancements.
The impact of polymeric gene delivery is expected to expand significantly with continued research and development.