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Trim23 promotes WSSV replication though negative regulation of antimicrobial peptides expression in Macrobrachium

Ruidong Zhang1, Xiaoling Dai1, Xueying Cao1

  • 1Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, College of Marine Science and Engineering, Nanjing Normal University, 1 Wenyuan Road, Nanjing, Jiangsu 210023, China.

Molecular Immunology
|June 26, 2020
PubMed

Insights

Macrobrachium nipponense Trim23 (MnTrim23) aids white spot syndrome virus (WSSV) infection by suppressing antimicrobial peptides. Inhibiting MnTrim23 reduces WSSV replication, highlighting its role in shrimp immunity.

Area of Science:

  • * Molecular Biology
  • * Immunology
  • * Zoology

Background:

  • * The TRIM (E3 ubiquitin ligase) protein family is crucial in vertebrate and invertebrate antiviral responses.
  • * White spot syndrome virus (WSSV) is a significant pathogen affecting shrimp aquaculture.

Purpose of the Study:

  • * To identify and characterize a novel TRIM protein, MnTrim23, from Macrobrachium nipponense.
  • * To investigate the role of MnTrim23 in WSSV infection and shrimp immune response.

Main Methods:

  • * Gene identification, protein domain analysis, phylogenetic analysis, and gene expression analysis (RT-qPCR).
  • * RNA interference (RNAi) for gene knockdown and analysis of WSSV replication and VP28 expression.
  • * Investigation of the regulatory relationship between MnTrim23, Relish, antimicrobial peptides (AMPs), and WSSV.

Main Results:

  • * A novel gene, MnTrim23, similar to Trim23, was identified in Macrobrachium nipponense, possessing conserved TRIM domains and an ARF domain.
  • * MnTrim23 expression was highest in the intestine and hepatopancreas and significantly upregulated in response to WSSV infection.
  • * Knockdown of MnTrim23 inhibited WSSV replication and VP28 expression, while MnTrim23 negatively regulated Relish-mediated AMP expression, and synthetic AMPs inhibited WSSV.

Conclusions:

  • * MnTrim23 plays a positive role in WSSV infection by inhibiting the expression of host antimicrobial peptides.
  • * The findings elucidate a novel antiviral mechanism involving MnTrim23 and AMPs regulated by the NF-κB pathway in shrimp.
Keywords:
Antimicrobial peptidesMacrobrachium nipponenseRelish transcription factorTrim23WSSV

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