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Immunohistochemical staining for terminal deoxynucleotidyl transferase (TDT). An enhanced method in routinely
J W Said1, I P Shintaku, G S Pinkus
1Department of Pathology and Laboratory Medicine, Cedars Sinai Medical Center, Los Angeles, California 90048.
Abstract:
This report describes an improved technique for sensitive and specific localization of terminal deoxynucleotidyl transferase (TdT) in routinely processed paraffin-embedded, formalin-fixed tissue sections using DNAse pretreatment and the avidin-biotin complex (ABC) technique. This method is useful in identifying lymphoblastic lymphomas (14/15 cases positive), with all other B- and T-cell lymphomas tested negative for the reaction. Used in conjunction with monoclonal antibodies immunoreactive for T- and B-cells in paraffin sections this technique should prove helpful in immunophenotyping malignant lymphomas where fresh tissue is unavailable for study.
