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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Dec 17, 2025

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

A high-throughput strategy for COVID-19 testing based on next-generation sequencing.

Jian Huang¹, Lan Zhao¹

¹Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL 60612, USA.

Medrxiv : the Preprint Server for Health Sciences

|June 27, 2020

Summary

This summary is machine-generated.

A new loop-mediated isothermal amplification (LAMP) assay offers rapid, cost-effective COVID-19 testing. This method enables precise diagnosis of thousands of specimens within days, improving public health decision-making for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

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Area of Science:

Molecular Biology
Virology
Diagnostic Assays

Background:

Accurate and timely COVID-19 testing is crucial for controlling severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread.
Current diagnostic methods like quantitative reverse-transcription PCR (qRT-PCR) face limitations in throughput, labor intensity, and accuracy, hindering widespread testing efforts.

Conclusions:

The developed LAMP-based strategy offers a scalable and economical solution for COVID-19 diagnosis.
This innovation can significantly enhance the capacity for SARS-CoV-2 testing, aiding public health responses.
The method's simplicity and efficiency support widespread implementation, even in resource-limited settings.