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Updated: Sep 7, 2026
In Situ Ca2+ Imaging of the Enteric Nervous System
Published on: January 29, 2015
Fluorescence digital image analysis of the inositol trisphosphate-mediated calcium transient in single permeabilized
Y Tsunoda1, S Yodozawa, Y Tashiro
1Department of Physiology, Kansai Medical University, Osaka, Japan.
Abstract:
The myo-inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ mobilization in single saponin-permeabilized and fura-2-loaded parietal cells was analysed by a fluorescence digital image processor. When the cells were incubated with ATP, free cytoplasmic Ca2+ concentration [( Ca2+]i) increased in some restricted cytoplasmic regions showing discontinuous plateau and in the peripheral cytoplasm showing continuous [Ca2+]i gradient towards the plasma membranes. When treated with IP3, the high plateau enlarged to the entire cytoplasm and (a) new higher plateau(s) appeared and enlarged again in a transient manner. The IP3-induced Ca2+ transient was also observed by fluorescence microphotometry of the single cells or by fluorescence spectrophotometry and 45Ca2+ uptake experiment of the cell population.
