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Updated: Dec 17, 2025

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
A fluorescent molecular rotor probe for tracking plasma membranes and exosomes in living cells
Ling Feng1, Yusheng Xie2, Sung King Au-Yeung3
1Cancer and Aging Research Institution, School of Life Science, Shandong University of Technology, China and Department of Chemistry and COSDAF (Centre of Super-Diamond and Advanced Films), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China. yushengxie@outlook.com hongysun@cityu.edu.hk and Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, P. R. China.
Abstract:
A rotor-based probe MRMP-1 was designed and synthesized. MRMP-1 can bind to plasma membranes very quickly and stably with remarkable fluorescence enhancement. It can be used to monitor the dynamic changes in cell membranes in real-time under stimuli conditions. Importantly, MRMP-1 is the first rotor-based fluorescent sensor to label exosomes in living cells.

