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A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC
Stefanie Brands1, Hannah U C Brass, Andreas S Klein
1Lehrstuhl für Biotechnologie, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany. u.schwaneberg@biotec.rwth-aachen.de.
Chemical Communications (Cambridge, England)
|June 27, 2020
Abstract:
A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries in Pseudomonas putida revealed a PigC variant that achieved a 2.9× increased yield of prodiginine derivatives.
