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Updated: Dec 17, 2025
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
Published on: March 9, 2017
Microfluidic Paper-Based Analytical Device for Histidine Determination
Akimitsu Kugimiya1, Akane Fujikawa2, Xiao Jiang3
1Department of Biomedical Information Sciences, Graduate School of Information Sciences, Hiroshima City University, 3-4-1 Ozuka-higashi, Asaminami-ku, Hiroshima, 731-3194, Japan. kugimiya@hiroshima-cu.ac.jp.
Abstract:
A laminated paper-based analytical device (LPAD) for histidine detection was fabricated from a chromatography filtration paper and laminate films. Histidine recognition was effected by histidyl-tRNA synthetase (HisRS), and its detection was signaled colorimetrically based on the molybdenum blue reaction. The analytical conditions and detectable concentration range of histidine were examined. The method provided selective quantification from 1 to 100 μM histidine. LPAD fabrication is considerably simple, involving only the craft-cutting of the chromatography filtration paper and laminate film, and is cost-effective.
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