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Updated: Dec 17, 2025

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
Published on: April 25, 2018
Promoter anchored interaction landscape of THP-1 macrophages captures early immune response processes
Sailendra Pradhananga1, Rapolas Spalinskas1, Flore-Anne Poujade2
1KTH Royal Institute of Technology, School of Chemistry, Biotechnology and Health, Science for Life Laboratory, Tomtebodavägen 23A, 171 65 Stockholm, Sweden.
Abstract:
Macrophages are highly plastic immune cells with temporally distinct transcriptome changes upon lipopolysaccride (LPS) activation. However, to what extent transcriptome reprogramming is mediated via spatial chromatin looping is not well studied. We generated high resolution chromatin interaction maps for LPS-stimulated THP-1 macrophages (0 and 2 h) using capture Hi-C. Success of LPS stimulation was validated with transcriptome sequencing. Circa 2900 genes changed their interaction profile upon LPS stimulation and those gaining interactions were enriched for LPS response relevant processes, suggesting a substantial role for distal regulation. Immune and cardiovascular risk variants were enriched within the interacting regions, thereby providing insights into macrophage biology.
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