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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Related Experiment Video

Updated: Dec 17, 2025

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

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Investigating Cellular Recognition Using CRISPR/Cas9 Genetic Screening.

Zheng-Shan Chong1, Gavin J Wright1, Sumana Sharma1

  • 1Cell Surface Signalling Laboratory, Wellcome Sanger Institute, Cambridge CB10 1SA, UK.

Trends in Cell Biology
|June 30, 2020
PubMed
Summary
This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats (CRISPR) screening advances the identification of cell surface molecules crucial for cellular recognition. This technology enables genome-wide discovery of molecular determinants in cell communication.

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Last Updated: Dec 17, 2025

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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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Area of Science:

  • Cell Biology
  • Genomics
  • Immunology

Background:

  • Cellular recognition, mediated by cell surface receptor-ligand interactions, is vital for biological processes like pathogen entry and immune responses.
  • Identifying molecular factors governing cell recognition at a genome-wide scale is a significant technical challenge.

Purpose of the Study:

  • To review the application of genome-scale clustered regularly interspaced short palindromic repeats (CRISPR) knockout/activation (CRISPR-KO/CRISPRa) screens in identifying cellular recognition determinants.
  • To discuss the contribution of CRISPR screening to understanding cell-cell communication and its potential in various biological contexts.

Main Methods:

  • Utilizing genome-scale CRISPR-KO/CRISPRa screening to identify genes involved in cellular recognition.
  • Reviewing existing literature on the application of CRISPR screening in cell surface recognition studies.

Main Results:

  • CRISPR-KO/CRISPRa screens have emerged as powerful tools for genome-wide identification of molecular determinants in cellular recognition.
  • These screening methods facilitate the discovery of key factors driving cell surface interactions.

Conclusions:

  • CRISPR screening significantly enhances our understanding of cellular recognition processes.
  • The application of CRISPR technology holds promise for investigating diverse cell-cell interactions across biological systems.