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A simple radioimmunoassay of human interleukin-2
T P Lee1, B K Mookerjee, J L Pauly
1Department of Medicine, Veterans Administration Medical Center, Buffalo, NY 14215.
Abstract:
We have developed a liquid phase radioimmunoassay of human interleukin-2 which uses inexpensive commercially available reagents exclusively. The assay is simple, reproducible and specific in detecting different batches of human interleukin-2 of natural as well as recombinant origin, but not detecting recombinant murine interleukin-2. The assay is sensitive to a concentration of approximately 0.05 ng/ml and can be used in measurement of IL-2 in serum containing culture media.