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Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

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Published on: May 16, 2022

Simultaneous Nanothermometry and Deep-Tissue Imaging.

Pascal M Gschwend¹, David Niedbalka^1,2, Lukas R H Gerken^3,4

¹Particle Technology Laboratory Institute of Process Engineering Department of Mechanical and Process Engineering ETH Zürich Sonneggstrasse 3 Zurich CH-8092 Switzerland.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|June 30, 2020

Summary

This summary is machine-generated.

New barium phosphate nanoparticles offer bright, stable, and biocompatible fluorescence for deep tissue imaging in the second biological window. These nanoparticles also enable temperature sensing, advancing biomedical imaging capabilities.

Keywords:

Mn5+‐doped Ba3(PO4)2 bioimaging cytocompatibility flame spray pyrolysis intravital microscopy

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Area of Science:

Nanotechnology
Biomedical Imaging
Materials Science

Background:

Fluorescent contrast agents are crucial for deep tissue imaging.
Agents operating in the second biological window (1000-1350 nm) are ideal for imaging deep structures like tumors.
There is a need for contrast agents that provide functional information, such as local temperature.

Purpose of the Study:

To develop and characterize novel barium phosphate nanoparticles as fluorescent contrast agents.
To evaluate their performance in deep-tissue imaging and their potential for nanothermometry.
To assess the stability and biocompatibility of these nanoparticles.

Main Methods:

Fabrication of water-dispersible barium phosphate nanoparticles doped with Mn5+ using flame aerosol technology.
Assessment of nanoparticle stability, toxicity (HeLa, THP-1, NHDF cell lines), and deep-tissue imaging capabilities (up to 3 cm).
Evaluation of temperature sensitivity and quantum yield of the nanoparticles.

Main Results:

Barium phosphate nanoparticles exhibit temperature-sensitive peak emission at 1190 nm in the NIR-II region.
High quantum yield (up to 34%) and excellent temperature sensitivity were achieved.
Demonstrated stability, low toxicity in representative cell lines, and successful deep-tissue imaging.
Concurrent deep-tissue imaging and nanothermometry are feasible with these nanoparticles.

Conclusions:

Scalable flame aerosol technology enables sterile production of Mn5+-doped barium phosphate nanoparticles.
These nanoparticles are promising biocompatible fluorescent contrast agents for deep-tissue imaging and nanothermometry.
The developed nanoparticles offer a dual-modality approach for advanced biomedical applications.