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Published on: March 31, 2021
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pH-Responsive Antibacterial Resin Adhesives for Secondary Caries Inhibition
1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, China.
Journal of Dental Research
|July 1, 2020
Summary
New pH-responsive tertiary amine-modified resin adhesives (TA@RAs) effectively prevent secondary dental caries. These intelligent materials exhibit reversible antibacterial effects, promoting oral eubiosis and improving dental restoration longevity.
Area of Science:
- Biomaterials Science
- Dental Materials
- Antimicrobial Materials
Background:
- Secondary caries significantly contribute to resin composite restoration failure.
- Existing antimicrobial dental materials lack intelligent response to oral environment changes.
- Maintaining oral eubiosis is crucial for preventing secondary caries.
Purpose of the Study:
- To develop novel pH-responsive tertiary amine-modified resin adhesives (TA@RAs) with anticaries properties.
- To investigate the reversible antibacterial and antibiofilm effects of TA@RAs in response to pH changes.
- To evaluate the efficacy of TA@RAs in preventing secondary dental caries in vitro and in vivo.
Main Methods:
- Synthesis of novel tertiary amine (TA) monomers: DMAEM and HMAEM.
- Incorporation of TA monomers into resin adhesives at 5% mass fraction to create TA@RAs.
- Evaluation of antibacterial activity against oral bacteria (e.g., *Streptococcus mutans*) in acidic and neutral conditions.
- Assessment of mechanical properties, biocompatibility, and antibiofilm efficacy using various in vitro and in vivo models.
- Analysis of microbial community diversity using 16S rRNA gene sequencing.
Main Results:
- TA@RAs demonstrated pH-responsive antibacterial activity, effective only in acidic conditions.
- The mechanical properties and biocompatibility of TA@RAs remained unaffected.
- TA@RAs exhibited reversible antibiofilm effects in response to pH fluctuations, mimicking oral de-/remineralization cycles.
- Long-term stability of antibacterial effect was confirmed through pH-cycling and biofilm aging models.
- 16S rRNA gene sequencing indicated that TA@RAs promote oral microbial diversity, suggesting a shift towards a healthier oral microbiome.
- In vitro and in vivo models confirmed the significant prevention of secondary dental caries by TA@RAs.
Conclusions:
- The developed TA@RAs offer a promising solution for preventing secondary caries.
- The pH-responsive, reversible antibacterial mechanism overcomes limitations of current dental materials.
- These novel resin adhesives hold significant potential for clinical application in restorative dentistry.
