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Near-Infrared Spectroscopy in Bio-Applications
Krzysztof B Beć1, Justyna Grabska1, Christian W Huck1
1Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 80/82, CCB-Center for Chemistry and Biomedicine, 6020 Innsbruck, Austria.
Molecules (Basel, Switzerland)
|July 2, 2020
Summary
Near-infrared (NIR) spectroscopy is a versatile vibrational technique for bioscience. Advances in instrumentation and analysis enhance its molecular insights and broad bio-application potential.
Area of Science:
- Bioscience and related disciplines
- Analytical Chemistry
- Molecular Spectroscopy
Background:
- Near-infrared (NIR) spectroscopy is a vibrational technique distinct from IR or Raman spectroscopy.
- It provides molecular information by measuring absorption bands from overtones and combination excitations.
- Significant advancements in instrumentation and spectral analysis have increased its applicability.
Purpose of the Study:
- To present NIR spectroscopy as a mature technique with substantial potential for bio-applications.
- To critically assess its practical value and compare it with competing spectroscopic methods.
- To connect NIR spectroscopy's bio-application potential with its fundamental characteristics.
Main Methods:
- Review of recent progress in NIR instrumentation (e.g., miniaturized spectrometers).
- Analysis of advanced spectral analysis methods (e.g., spectral image processing, quantum chemical calculations).
- Critical assessment and comparison with other spectroscopic techniques (IR, Raman).
Main Results:
- NIR spectroscopy is a well-established technique with ongoing advancements.
- Its molecular elucidation capabilities are enhanced by modern instrumentation and analysis.
- The technique shows great potential for diverse bio-applications.
Conclusions:
- NIR spectroscopy offers unique advantages for bioscience due to its fundamental characteristics.
- Continued development promises expanded applications in various biological fields.
- Its practical value is significant and competitive with other spectroscopic methods.
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