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Ceria-Containing Hybrid Multilayered Microcapsules for Enhanced Cellular Internalisation with High Radioprotection
N R Popova1, A L Popov1, A M Ermakov1
1Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia.
Molecules (Basel, Switzerland)
|July 2, 2020
Summary
Polyelectrolyte microcapsules loaded with cerium oxide nanoparticles (nanoceria) offer controlled intracellular delivery and radioprotective effects for human mesenchymal stem cells. This study elucidates the mechanisms behind nanoceria
Area of Science:
- Biomedical Nanotechnology
- Cell Biology
- Radioprotection
Background:
- Cerium oxide nanoparticles (nanoceria) are promising nanozymes for biomedical use.
- Controlled intracellular delivery of nanoceria is a significant challenge.
- Understanding nanoceria's protective mechanisms against radiation is crucial.
Purpose of the Study:
- To develop polyelectrolyte microcapsules for controlled intracellular delivery of nanoceria.
- To evaluate the radioprotective and genoprotective effects of nanoceria-loaded microcapsules in human mesenchymal stem cells.
- To elucidate the molecular mechanisms underlying nanoceria's protective action.
Main Methods:
- Fabrication of polyelectrolyte microcapsules loaded with cerium oxide nanoparticles.
- Assessment of cell viability and uptake efficiency at various cell-to-capsule ratios.
- Measurement of intracellular reactive oxygen species (ROS) levels.
- Gene expression analysis of 96 key genes related to oxidative stress, metabolism, apoptosis, and inflammation.
- Evaluation of cytogenetic damage in irradiated cells.
Main Results:
- Optimal concentrations for safe and efficient uptake of ceria-containing microcapsules were determined (1:10 to 1:20 cell-to-capsules ratio).
- Nanoceria demonstrated a radioprotective effect by reducing intracellular ROS and modulating gene expression.
- Hybrid microcapsules provided an indirect genoprotective effect, decreasing cytogenetic damage in irradiated cells.
Conclusions:
- Polyelectrolyte microcapsules enable controlled loading and intracellular release of nanoceria.
- Nanoceria-loaded microcapsules offer significant radioprotective and genoprotective benefits against ionizing radiation.
- These findings advance the understanding of nanoceria's application in protecting biological systems from radiation damage.
Keywords:
cerium oxide nanoparticlesintracellular deliverynanoceriaoxidative stresspolyelectrolyte microcapsulesradioprotectionMore Related Videos
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