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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Recent Advances in Microfluidic Paper-Based Analytical Devices toward High-Throughput Screening.

Siraprapa Boobphahom1, Mai Nguyet Ly2, Veasna Soum2

  • 1Metallurgy and Materials Science Research Institute, Chulalongkorn University, Soi Chula 12, Phayathai Road, Pathumwan, Bangkok 10330, Thailand.

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|July 2, 2020
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Summary

Microfluidic paper-based analytical devices (µPADs) offer cost-effective, portable solutions for point-of-care diagnostics. Recent advancements focus on high-throughput, multiplexed assays for diverse applications.

Keywords:
biomarkersdrug analysisenvironmental monitoringfood quality controlimmunoassayinfectious diseasesmicrofluidic paper-based analytical devices

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Materials Science

Background:

  • Microfluidic paper-based analytical devices (µPADs) are cost-effective, portable tools for point-of-care (POC) chemical and biological assays.
  • µPADs offer advantages over traditional devices, including ease of fabrication and disposability.

Purpose of the Study:

  • This review focuses on recent advancements in µPADs to achieve high-throughput capabilities.
  • The study discusses fabrication techniques, designs, and detection methods for multiplexed assays.

Main Methods:

  • Review of existing literature on µPAD fabrication techniques and designs.
  • Analysis of current detection methods for multiplexed assays.
  • Discussion of applications in clinical diagnosis, drug analysis, environmental monitoring, and food quality control.

Main Results:

  • Recent µPAD developments enable multistep assays, high sensitivity, and rapid readout.
  • µPADs are increasingly utilized for parallel analyses to detect biomarkers.
  • The review highlights progress towards high-throughput and multiplexed detection using µPADs.

Conclusions:

  • µPADs are evolving into powerful tools for high-throughput, multiplexed analyses across various fields.
  • Future perspectives suggest continued innovation in µPAD design and application.
  • µPADs are poised to significantly impact POC diagnostics and other analytical areas.