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Localized epileptiform activity induced by murine CRF in rats
F Marrosu1, W Fratta, P Carcangiu
1Institute of Neurology, University of Cagliari, Italy.
Epilepsia
|July 1, 1988
Summary
Murine corticotropin releasing factor (rCRF) induced epileptic activity in rats, which was reduced by carbamazepine. This rCRF-induced spiking may serve as a model for temporal lobe epilepsy research.
Area of Science:
- Neuroscience
- Epileptology
Background:
- Corticotropin-releasing factor (CRF) is implicated in stress responses.
- CRF's role in epilepsy is not fully understood.
Purpose of the Study:
- To investigate the effects of rat CRF (rCRF) on brain activity in rats.
- To determine if rCRF-induced activity could model temporal lobe epilepsy.
- To assess the efficacy of carbamazepine and naloxone in mitigating rCRF effects.
Main Methods:
- Intracerebroventricular injection of rCRF (10 micrograms) in rats.
- Monitoring of hippocampal electroencephalogram (EEG) for epileptic activity.
- Administration of carbamazepine (15 and 30 mg/kg) and naloxone (8 mg/kg) via intraperitoneal injection.
Main Results:
- rCRF injection increased motor activity, grooming, and induced recurrent hippocampal epileptic-like activity lasting approximately 5 hours.
- This activity consisted of biphasic spikes without behavioral epilepsy signs.
- Carbamazepine reduced epileptic activity for 90-120 minutes, while naloxone was ineffective.
Conclusions:
- rCRF induces a distinct form of epileptic activity in rats.
- This rCRF-induced spiking activity shows potential as an animal model for temporal lobe epilepsy.
- Carbamazepine demonstrates anticonvulsant properties against this induced activity.