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Staphylococcus pseudintermedius 5'-nucleotidase suppresses canine phagocytic activity
Mohamed A Abouelkhair1, Linda A Frank2, David A Bemis3
1Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN, USA; Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Menoufia, Egypt.
Veterinary Microbiology
|July 2, 2020
Summary
Staphylococcus pseudintermedius evades immune cells using the SpAdsA protein. Inhibiting SpAdsA or using antibodies against it may lead to new vaccines for canine pyoderma.
Area of Science:
- Veterinary Microbiology
- Immunology
- Bacterial Pathogenesis
Background:
- Staphylococcus pseudintermedius is a leading cause of canine pyoderma and other infections.
- Antimicrobial resistance necessitates alternative treatment strategies, including vaccines.
- Identifying virulence factors is crucial for developing effective staphylococcal vaccines.
Purpose of the Study:
- To identify and characterize virulence factors of Staphylococcus pseudintermedius.
- To investigate the role of a novel 5'-nucleotidase, SpAdsA, in bacterial virulence.
- To evaluate SpAdsA as a potential vaccine candidate against S. pseudintermedius infections.
Main Methods:
- Identified and characterized a putative 5'-nucleotidase (SpAdsA) in S. pseudintermedius.
- Assessed the enzymatic activity of SpAdsA and its role in virulence.
- Investigated the effects of adenosine, SpAdsA inhibitors, and receptor antagonists on phagocytosis and bacterial killing.
- Evaluated the neutralizing capacity of antibodies against SpAdsA.
Main Results:
- SpAdsA, a cell-wall protein, was identified and shown to have 5'-nucleotidase activity.
- Exogenous adenosine inhibited phagocytosis of S. pseudintermedius by canine immune cells.
- Inhibiting SpAdsA or its receptor (A2A) enhanced bacterial killing by phagocytic cells.
- Antibodies against SpAdsA demonstrated neutralizing potential.
Conclusions:
- SpAdsA is a significant virulence factor for Staphylococcus pseudintermedius, aiding immune evasion.
- Targeting SpAdsA offers a promising strategy for combating S. pseudintermedius infections.
- Attenuated SpAdsA is a potential candidate for a novel vaccine against canine pyoderma and related infections.
