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Updated: Dec 16, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
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Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

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Down-Regulation of Ribosomal Protein RPS21 Inhibits Invasive Behavior of Osteosarcoma Cells Through the Inactivation

Tao Wang1, Zhi-Yong Wang1, Ling-Yuan Zeng1

  • 1Department of Orthopedics, The Second Hospital of Shanxi Medical University, Taiyuan City, Shanxi Province 030001, People's Republic of China.

Cancer Management and Research
|July 3, 2020
PubMed
Abstract

Insights

Ribosomal protein S21 (RPS21) is upregulated in osteosarcoma (OS) and promotes cancer progression. Inhibiting RPS21 may offer a new therapeutic strategy for OS by impacting the MAPK pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma (OS) is a primary bone malignancy with significant morbidity.
  • Understanding the molecular drivers of OS is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of ribosomal protein S21 (RPS21) in human osteosarcoma (OS).
  • To explore RPS21's expression, function, and molecular mechanisms in OS.

Main Methods:

  • RPS21 expression was analyzed in OS tissues and cell lines using GEO datasets and qRT-PCR.
  • RPS21 was silenced using siRNA, and its effects on OS cell proliferation, migration, and invasion were assessed via CCK-8, colony formation, wound-healing, and Transwell assays.
  • Western blot analysis was used to examine the impact on MAPK signaling pathway proteins.

Main Results:

  • RPS21 expression was significantly elevated in OS tissues compared to normal samples.
  • Higher RPS21 levels correlated with poorer patient outcomes.
  • Silencing RPS21 reduced OS cell proliferation, colony formation, migration, and invasion.
  • Downregulation of RPS21 led to the inactivation of the MAPK signaling pathway.

Conclusions:

  • RPS21 functions as an oncogene in OS development.
  • RPS21 regulates OS progression through the MAPK pathway.
  • RPS21 presents a potential therapeutic target for osteosarcoma treatment.
Keywords:
MAPK pathwayRPS21migrationosteosarcomaviability

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