New morphological features based on the Sholl analysis for automatic classification of traced neurons

This article addresses the automatic classification of reconstructed neurons through their morphological features. The purpose was to extend the capabilities of the L-Measure software.

New morphological features were developed, based on modifications of the conventional Sholl analysis. The lengths of the compartments, as well as their volumes, were added to the features used in the classical analysis in order to improve the results during automatic neuron classification. FSM were used to obtain subsets of lower cardinality from the full feature sets and the usefulness of these subsets was tested through their use in supervised classification tasks. The study was based on two types of neurons belonging to mice: pyramidal and GABAergic interneurons. Furthermore, a set of pyramidal neurons belonging to Later 4 and Layer 5 was analyzed.

RF classifier shown the best performance combined with a Wrapper method.U-WNAD set allowed to obtain higher values than WN, A and D in all cases and better results than LM for the filters and wrappers FSM. U-LM-WNAD set, led to the highest AUC values for all the FSM studied. Similar results for different regions of cortex were obtained. Comparison with Existing Methods The new features exhibited high discriminatory power with which the values of AUC and Acc obtained in the experiments exceeded those obtained using only the features provided by L-Measure.

The highest values of AUC and Acc were obtained from the sets U-WNAD and U-LM-WNAD, evidencing the discriminatory power of the new proposed features.